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,青青草在线七次郎-青青草视频免费寓目
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心理旌旗灯号丈量

神经递质检测体系

工夫:2016-09-27青青草在线七次郎-青青草视频免费寓目作者:玉研仪器

,青青草在线七次郎-青青草视频免费寓目

具体引见

神经递量及时检测体系运用快速扫描轮回伏安法(FSCV)手艺,快速及时监测植物体内的儿茶酚胺类神经化学物质的含量(如多巴胺,去甲肾上腺素,血清素等)。体系运用了碳纤维生物传感器,可正在大小鼠身材上实现短时间的快速丈量。


体系有两种式子:一种为用于大鼠的无线遥测体系,一种为应用到小鼠的有线遥测体系。,青青草在线七次郎-青青草视频免费寓目


技术优势:
√ 优越的空间分辨率-直径34um 碳纤维
√ 优越的时间分辨率- 5-10 sweeps/s
 准确度-运用现有的可定义的明白儿茶酚胺类符号物,扫除了其他品种物资的滋扰。
√ 采样速度: 5k/s (大鼠体系),大概10K/s(小鼠体系)

事情道理:
生物胺类物资的含量经由过程快速轮回性的施加电压到一根植入式碳纤维传感器,然后检测响应的发生的电流转变停止测定。体系使得正在停止具体的行为学研讨的同时,能够丈量自觉发生的亚秒级的神经递量开释事宜。无论是有线照样无线体系,皆能够正在可选的量程(-0.6-+1.5V)范围内,停止250-400V/s的扫描检测。一切体系同时借内置支撑内部刺激的掌握。


软件:

体系配套软件不只支撑传统的短时间丈量形式(纪录2分钟之内的数据),同时借支撑扩大的一连临时纪录形式。除此之外,本软件的特性借包孕配景乐音消弭,热点图,3D可视化视图,可选的滤波和静态的伏案图。数据能够导出为通用的EXCEL格式文件。别的,软件借支撑整合同步视频捕获,以便适合于植物行动取生物物资开释干系的同步研讨。99久久免费热在线佳构_久久re6热在线佳构视频_久久99re热在线播放


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Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals.





HOW IT WORKS

Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V/s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.

l  Voltage Span: -1.1 V to +1.3 V

l  Range: 250 – 400 V/s 

l Sweeps/second: 5 - 10 

l Points/sweep: 1000 



Analysis

Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.


The TETHERED FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function. A headmounted FSCV board sends signals through a low-torque commutator to an interface box that streams data to the host PC. The system comes with Pinnacle’s FREE 8500 software.



1. The FSCV interface box provides access to stimulus lines and transmits data to PAL-8500 software.

2. A low-torque commutator, which is mounted above the cage, allows for unencumbered freedom of movement.

3. Signals are amplified and filtered at the head of the animal using our headstage, which ensures the delivery of clean, artifact-free data.

4. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors. Headmounts are affixed to the skull with dental acrylic and act as a connection port for the headstage.

 




CARBON FIBER


Pinnacle’s FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 µm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag/AgCl reference electrode.


Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

 




CARBON FIBER SENSORS

Carbon fiber sensors are ordered by cannula type and whether the researcher needs to remove them from the cannula for post-calibration. CFS-F sensors are fixed in the cannula and cannot be removed for post-calibration。



FSCV PAL-8500 SOFTWARE PACKAGE

The FSCV system includes Pinnacle’s FREE PAL-8500 software, which supports traditional, short recording paradigms (recordings of two minutes or less) as well as longer-term recordings that use an extended continuous mode. Furthermore, the suite supports integrated, synchronized video recording, which allows monitoring of animal behavior simultaneously with biogenic amine release.

 


Additional features of this software include:
• Background Subtraction
• 3D Visualization 
• User-Selectable Filters 
• Heat Maps 
• Animated Voltammograms
• Export to Third-Party Packages 




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